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Published on: March 17, 2023
Femtosecond Laser-Fabricated Bowl-Shaped Surface-Enhanced Raman Scattering Platform for Multibiomarker Analysis of
Mingyang Han1, Xiao Yang1, Rui Guo1
1Hebei Key Laboratory of Materials Near-Net-Forming Technology, School of Material Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
Abstract:
Biomarkers for acute myocarditis (AM) are commonly used in clinical diagnosis, but their ultralow concentrations in blood are challenging to be detected in the early stage. This study presents a novel bowl-shaped surface-enhanced Raman scattering (SERS) substrate with high-performance, prepared via one-step hydrofluoric acid etching of SiO2 microspheres, 515 nm femtosecond-laser reduction of gold ions, and nanosecond-laser reshaping of gold nanoparticles (AuNPs). In particular, 500 nm SiO2 microspheres were etched with a hydrofluoric acid solution to form a uniform microbowl array, and AuNPs were synthesized in situ on the SiO2 microbowls via irradiation with a femtosecond laser. Subsequently, the AuNPs were reshaped into nanorods using a nanosecond laser. The resulting bowl-shaped AuNP@SiO2 substrate showed a SERS enhancement factor of 106 and a limit of detection of 3.84 × 10-8 M, as evaluated on Rhodamine 6G. Notably, the limits of detection of the AM biomarkers were lower than 6 pg/mL, enabling the accurate detection of their trace quantities. For AM prediction, a fuzzy logic model was trained, enabling the accurate and quantitative analysis of biomarkers, with a mean relative error of less than 25.7%. Thus, the proposed SERS platform is a reliable tool for the early noninvasive diagnosis of AM.
