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Published on: December 8, 2016
Benchmark Comparison of Bone Marrow Aspiration Systems: Increased Progenitor Cell Concentrations Achieved Without
Anthony Scillia1, Teo Mendez2, Dan Kuebler3
1Sports Medicine/Orthopedics, Seton Hall University, Paterson, USA.
Cureus
|July 13, 2026
Summary
A novel bone marrow aspiration system (B-MAN™) effectively captures high concentrations of mesenchymal stromal cells (MSCs) and hematopoietic progenitors without centrifugation. This method minimizes hemodilution, offering a promising advancement for regenerative medicine and orthopedic procedures.
Area of Science:
- Orthopedics and Regenerative Medicine
- Biomedical Engineering
- Cellular Biology
Background:
- Bone marrow aspirate (BMA) is crucial for orthopedic and regenerative procedures, containing mesenchymal stromal cells (MSCs) and hematopoietic progenitors vital for tissue repair.
- Conventional aspiration methods suffer from hemodilution, reducing cell concentrations and increasing sample variability.
- Hemodilution occurs as peripheral blood contaminates the aspirate drawn from a fixed site, decreasing the yield of valuable MSCs and progenitors.
Purpose of the Study:
- To evaluate the cell yield of the novel B-MAN™ Bone Marrow Aspiration System under standardized conditions.
- To compare the B-MAN™ system's performance against published data for centrifuge-processed bone marrow concentrate (BMAC) and other no-spin aspiration devices.
- To assess the impact of aspiration technique on mesenchymal stromal cell and progenitor cell concentrations in bone marrow aspirate.
Main Methods:
- Retrospective analysis of bone marrow aspirate from five adult males undergoing elective orthopedic procedures using the B-MAN™ system.
- Aspirations of 1 mL were performed from the anterior iliac crest via a single cortical access site without centrifugation.
- Laboratory analysis included total nucleated cell (TNC) concentration, CD34+ cell count, colony-forming unit-fibroblast (CFU-f) concentration, and cell viability.
Main Results:
- The B-MAN™ system yielded high mean concentrations: 102 × 10⁶ TNC/mL, 15,145 CFU-f/mL, and 1,143,216 CD34+ cells/mL, with 96.9% viability.
- The mean CFU-f:TNC ratio was 0.013%, aligning with or exceeding the upper range for minimally diluted marrow, indicating low hemodilution.
- These results position B-MAN™ aspirates among the highest concentrations reported for both centrifuge-processed BMAC and optimized no-spin systems.
Conclusions:
- The B-MAN™ system effectively produces unprocessed marrow aspirate with high concentrations of progenitor cells.
- These findings suggest that aspiration interface engineering is a valuable strategy for mitigating hemodilution.
- Further research into aspiration interface design is warranted to enhance cell yield and consistency in bone marrow aspirate for regenerative applications.
