Related Experiment Video
Updated: Jul 14, 2026
10:34
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Published on: August 20, 2012
A ratiometric FRET sensor regulated by amide naphthotubes via simultaneous spatial confinement and spectral red-shift
Yu-Ke Liu1, Jia-Yao Ren1, Ya-Qing Cui1
1School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China. yanglp@usc.edu.cn.
Chemical Communications (Cambridge, England)
|July 13, 2026
Abstract
None:
A ratiometric FRET sensor using amide naphthotubes (NTs) is reported. A red-shift of the acceptor styrylpyridinium dyes absorption is induced upon NT binding, aligning it with the BODIPY donor emission without crosstalk. FRET is turned off by analyte displacement. Six β-blockers are detected (LOD = 0.23-0.51 µM) and discriminated in buffer and serum.
