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Updated: Jul 15, 2026
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
Tailor-made versatile and efficient gold-based nanomaterials: From synthesis, properties to their application in
Guoyang Xie1, Weiqiang Li2, Gan Zhang2
1Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China.
None:
Lateral flow assays (LFAs) are regarded as point-of-care biosensors that enable the rapid detection of targets based on signal changes. Traditional 13-nm colloidal gold nanoparticles have historically played an essential role in LFA. In recent years, compared with traditional colloidal gold, gold-based nanomaterials (GBNMs) have become a research hotspot because of their attractive properties. Starting from GBNMs, this study explores their structures, synthesis, and properties. We provide a detailed overview of four different types of GBNMs used in LFA and their main physicochemical properties, including colorimetric response, photothermal effect, fluorescence emission, and nanozyme activity. Subsequently, based on antigen-antibody or aptamer-based recognition formats, GBNMs are integrated with various assay formats to achieve rapid target identification. Different types of LFA are classified according to the number of targets and signal readers and are designated as "1 + 1", "1 + n", "m + 1", and "m + n" (m ≥ 2, n ≥ 2), respectively. Various GBNM-based LFA biosensors have been used to detect different hazardous factors closely related to human health. In addition, several new tools integrated with LFA have also been investigated. Finally, we discuss the current challenges and prospects in detail.
