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A Biologically Active Yellow Pigment From Micrococcus luteus (PX673990), Production, Extraction, Purification,
Minakshi Chakraborty1, Royee Santra1, Jayati Bhowal1
1School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, Howrah, West Bengal, India.
Biotechnology and Applied Biochemistry
|July 14, 2026
Summary
Micrococcus luteus produced a yellow bio-pigment from orange peel waste. This sustainable dye shows potential for textiles and paper, offering a cost-effective alternative to synthetic dyes.
Area of Science:
- Microbiology
- Biotechnology
- Materials Science
Background:
- Synthetic dyes pose environmental and health risks.
- Microbial pigments offer sustainable and safer alternatives.
- Orange peel waste is an abundant, low-cost substrate.
Purpose of the Study:
- To produce and characterize bio-pigments from Micrococcus luteus using orange peel waste.
- To evaluate the pigment's potential as a sustainable dye for industrial applications.
- To investigate the pigment's chemical structure and antioxidant properties.
Main Methods:
- Cultivation of Micrococcus luteus on orange peel waste.
- Partial purification of the microbial pigment.
- Characterization using HPLC, FTIR, 1H, 13C NMR, and XRD.
- Evaluation of dyeing properties on textiles and paper.
- Assessment of antioxidant activity (DPPH and ABTS assays).
Main Results:
- A yellow bio-pigment was successfully produced and partially purified.
- The pigment is a glycosylated carotenoid-like compound with esterified fatty acids.
- High crystallinity and promising dyeing properties were observed.
- Significant antioxidant activity was demonstrated (42.44% DPPH, 37.25% ABTS).
Conclusions:
- Micrococcus luteus can efficiently produce bio-pigments from orange peel waste.
- The characterized bio-pigment shows potential as a sustainable, cost-effective dye for textiles and paper.
- This microbial pigment offers a viable alternative to synthetic dyes, reducing environmental impact.
