Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fast fixed-time prescribed performance adaptive control for multi-aircraft formation reconfiguration based on BELNN disturbance observer.

ISA transactions·2026
Same author

Model-Assisted Process Optimization for Trivalent Ligand-siRNA Conjugation via CuAAC Click Chemistry.

ACS omega·2025
Same author

Synthesis of DNA-Encoded Bicyclic Peptides via Cysteine-Promoted Cyclization and Amide Condensation Reaction.

Bioconjugate chemistry·2025
Same author

Deep seeking covalent DNA encoded library for novel JAK3 inhibitor discovery.

Bioorganic & medicinal chemistry·2025
Same author

Chemically Induced Nuclear Pore Complex Protein Degradation via TRIM21.

ACS chemical biology·2025
Same author

Identification of TIGAR, a direct proteomic target associated with the hypoglycemic effect of Berberine.

Fitoterapia·2024

Related Experiment Video

Updated: Jul 15, 2026

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

A fast high-throughput logP estimation method for peptide molecules based on liquid chromatography-mass spectrometry

Jing Deng1, Hengmao Zhang1, Yixuan Zhai1

  • 1HitGen Inc., Shuangliu District, Chengdu, Sichuan, 610200, P. R. China.

Medicinal Chemistry Research : an International Journal for Rapid Communications on Design and Mechanisms of Action of Biologically Active Agents
|July 14, 2026
PubMed
Summary

We developed a high-throughput protocol for rapid logP estimation in peptide molecules using pool and split synthesis and UPLC-MS. This method significantly accelerates lead molecule screening in drug development.

Keywords:
High-throughput synthesisLogPPeptideUPLC-MS

More Related Videos

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Related Experiment Videos

Last Updated: Jul 15, 2026

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Area of Science:

  • * Pharmaceutical Sciences
  • * Analytical Chemistry
  • * Medicinal Chemistry

Background:

  • * Accurate prediction of lipophilicity (logP) is crucial for drug discovery.
  • * Traditional methods for logP determination are often low-throughput and time-consuming.
  • * High-throughput screening requires efficient and reliable methods for assessing molecular properties.

Purpose of the Study:

  • * To develop a complete end-to-end high-throughput protocol for rapid estimation of peptide logP values.
  • * To integrate high-throughput synthesis with advanced analytical techniques for efficient sample analysis.
  • * To establish a robust correlation model for automated data analysis and accelerate drug discovery pipelines.

Main Methods:

  • * Utilized "pool and split" high-throughput synthesis for efficient peptide sample preparation.
  • * Developed a logP detection method employing ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS).
  • * Established a linear correlation model between chromatographic capacity factor (logK') and logP (R² = 0.92) for automated analysis.

Main Results:

  • * Successfully synthesized and detected mixed samples containing 16, 81, and 256 peptide molecules.
  • * Achieved a logP detection rate exceeding 85% for the peptide molecules.
  • * Demonstrated a sample processing capacity exceeding 20,000 samples per day.

Conclusions:

  • * The developed protocol offers a rapid and efficient method for estimating peptide logP values.
  • * This high-throughput system significantly accelerates the early screening of lead molecules in drug development.
  • * The integration of synthesis, detection, and automated analysis provides a powerful tool for medicinal chemistry research.