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Updated: Jul 16, 2026
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Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles
Published on: November 14, 2025
Multidimensional strategies for human milk extracellular vesicle preservation: a systematic evaluation based on
Chao Zhang1, Minzhi Zhou1, Qingli Mao1
1State Key Laboratory of Food Science and Resource, Nanchang University, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang 330047, China.
Food Chemistry
|July 14, 2026
Summary
Preserving human milk-derived extracellular vesicles (HM-EVs) is crucial. This study compared liquid, solid, and semi-solid formulations, finding trehalose/Pluronic® F-68 liquid offers superior room-temperature stability for HM-EVs.
Area of Science:
- Biotechnology
- Materials Science
- Biomedical Engineering
Background:
- Long-term preservation of extracellular vesicles (EVs) is a significant hurdle for their widespread use.
- Existing studies lack systematic comparisons of different physical-state formulations for human milk-derived EVs (HM-EVs).
Purpose of the Study:
- To systematically evaluate and compare liquid, lyophilized (solid), and hydrogel (semi-solid) formulations for preserving HM-EVs.
- To assess the impact of different formulations on particle integrity, cargo retention, and storage stability.
Main Methods:
- Developed liquid, lyophilized, and hydrogel formulations using HM-EVs from a single source.
- Compared preservation performance against commercial products under standardized conditions.
- Analyzed particle integrity, cargo retention, and storage stability at 4°C and 25°C for up to 3 months.
Main Results:
- The trehalose/Pluronic® F-68 liquid formulation demonstrated excellent room-temperature stability.
- The trehalose/Pluronic® F-68/mannitol lyophilized formulation provided effective short-term ambient storage preservation.
- Hydrogel formulation created a protective microenvironment suitable for short-term HM-EV applications.
Conclusions:
- Established a cross-format preservation framework for HM-EVs.
- The findings support the broader application of HM-EVs in functional foods and biomedicine.
- Different formulations offer distinct advantages for specific storage durations and conditions.
