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Related Experiment Video

Updated: Jul 16, 2026

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

CD4⁺ T cells persist with their Eomes.

Abishek Vaidya¹, Alice O Kamphorst²

¹Department of Immunology and Immunotherapy and Marc and Jennifer Lipschultz Precision Immunology Institute, ISMMS, New York, NY, USA; Graduate School of Biomedical Sciences, ISMMS, New York, NY, USA.

|July 14, 2026

Summary

Stem-like CD4+ T cells are crucial for sustained immune responses. Eomesodermin (EOMES) is identified as a key regulator essential for maintaining and differentiating these vital immune cells.

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Last Updated: Jul 16, 2026

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

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Published on: June 22, 2016

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In Vitro Resident Memory CD8 T Cell Differentiation Using Epithelial Organoid-T Cell Co-culture System

Published on: February 3, 2026

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

Stem-like CD4+ T cells, analogous to CD8+ T cells, play a critical role in adaptive immunity.
These cells are essential for mounting and sustaining responses against chronic infections, tumors, and inflammatory conditions.

Purpose of the Study:

To identify key regulators involved in the maintenance and differentiation of stem-like CD4+ T cells.
To elucidate the molecular mechanisms governing the function of these important immune cells.

Main Methods:

The study likely involved in vivo and in vitro experiments using mouse models and primary cell cultures.
Techniques may include flow cytometry, gene expression analysis, and functional assays to assess T cell populations and their functions.

Main Results:

Wen et al. and Agesta et al. independently identified Eomesodermin (EOMES) as a critical factor.
EOMES was shown to be indispensable for the maintenance of the stem-like phenotype in CD4+ T cells.
The research demonstrated EOMES's role in regulating the differentiation pathways of these cells.

Conclusions:

EOMES is a pivotal transcription factor for the biology of stem-like CD4+ T cells.
Understanding EOMES's function provides new insights into immune memory and T cell responses in chronic diseases and cancer immunotherapy.