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Updated: Jul 16, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
Published on: April 21, 2022
Epitranscriptomic Analysis of A-to-I RNA Editing and m6A Using Short- and Long-Read Sequencing Technologies
Nicholas Brenna1, Domenico Alessandro Silvestris2, Silvana Zugaro1
1Department of Onco-Haematology, Genetics and Epigenetics of Pediatric Tumours, Bambino Gesù Children's Hospital IRCCS, 00146 Rome, Italy.
None:
More than 160 types of post-transcriptional RNA modifications have been identified, revealing considerable diversity in their types, abundances, distributions, and functional roles across different RNAs, cells, and tissues in humans. Recent advances in high-throughput sequencing technologies have enabled the systematic detection of dynamic RNA modifications, including N6-methyladenosine (m6A) and inosine (I). In this review, we focus on RNA modifications in eukaryotic mRNA and provide an overview of current high-throughput methodologies for detecting the most abundant adenosine-related modifications, including m6A and adenosine-to-inosine (A-to-I) RNA editing. Finally, we discuss the major challenges that remain in the field and highlight key directions for future research.
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