Formulation-Process Screening of a High-Oil-Fraction Ovotransferrin-Lysozyme Concentrated Dispersion Stabilized by...

Ovotransferrin (OVT) and lysozyme (LYS) are food-derived proteins with reported bioactive properties, but results from high-oil-fraction dispersions require cautious interpretation when phase type and undiluted particle size are not independently verified. This work screened a low-temperature OVT-LYS concentrated dispersion in which freeze-drying was omitted from the final preparation stage. LYS was first incorporated into a β-cyclodextrin/trehalose-protected LYS complex (LPC). A sequential one-factor-at-a-time design was used to screen hydrocolloid type, homogenization parameters, and flaxseed gum/xanthan gum mass ratio. Qualitative optical microscopy, zeta potential, creaming stability, pH/temperature tolerance, OVT immunoreactivity, and DLS data obtained after 1000-fold dilution were used as descriptors. Among the six tested hydrocolloids, flaxseed gum (FG) showed the most favorable overall single-hydrocolloid performance. High-pressure homogenization at 1000 bar for 1.5 min, corresponding to approximately three estimated equivalent passes, was selected as a practical processing condition within the tested range. A flaxseed gum:xanthan gum mass ratio of 1:2 showed the most favorable balance between pH/temperature tolerance and OVT immunoreactivity retention among the tested binary blends. Because LYS bioactivity assays, phase-type verification, DLS dilution-gradient validation, and undiluted particle-size measurement were not performed, the results support bounded formulation-screening evidence, rather than verified phase structure, retained LYS activity, or universal optimal parameters.