Related Experiment Video
Updated: Jul 16, 2026

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
Substrate-Driven Modulation of Nutritional Composition and Bioactive Compound Profile in Pleurotus pulmonarius
Monika Kalinowska1,2, Marzena Smolewska1, Ewelina Gołębiewska1
1Department of Chemistry, Biology and Biotechnology, Faculty of Civil Engineering and Environmental Science, Institute of Civil Engineering and Energetics, Bialystok University of Technology, Wiejska 45E Street, 15-351 Bialystok, Poland.
Abstract:
Pleurotus pulmonarius (Fr.) Quél. is a commercially important edible mushroom recognized for its nutritional and nutraceutical value. However, the influence of alternative agricultural waste substrates on its biochemical composition remains insufficiently characterized. This study investigated the effect of four cultivation substrates, coconut waste (PpC), paddy husk (PpP100), paddy husk supplemented with sawdust (PpP20) and rubberwood sawdust as control (PpS), on the macro- and microelement profile, secondary metabolite composition, and antioxidant activity of P. pulmonarius fruiting bodies. Analytical methods included ICP-MS and FAAS for elemental analysis; GC-MS for fatty acid, carbohydrate and phenolic profiling; Kjeldahl method for total protein; FTIR spectroscopy for structural characterization; and four complementary antioxidant assays (DPPH, ABTS, CUPRAC, FRAP). Coconut waste substrate promoted the highest protein accumulation and elevated concentrations of iron, zinc, and specific phenolic acids (vanillic, protocatechuic). Paddy husk-based substrates favored carbohydrate accumulation, particularly trehalose, while sawdust supported the greatest lipid content, dominated by linoleic acid. Potassium was the predominant macroelement across all variants. Antioxidant activity was highest in PpP100 and PpC across all four assays. FTIR confirmed a mushroom-specific polysaccharide, protein, and lipid profile in all samples. The results demonstrate that agricultural waste represents sustainable, value-added alternatives to conventional sawdust, capable of maintaining or enhancing the nutritional and nutraceutical quality of oyster mushrooms to enhance agricultural production.
More Related Videos
09:39Production and Testing of Moisture Behavior and Thermal Properties of Rapeseed Straw and Ganoderma resinaceum Mycelium Bio-Composites
Published on: September 5, 2025
10:10Mass Production of Entomopathogenic Fungi, Metarhizium robertsii and Metarhizium pinghaense, for Commercial Application Against Insect Pests
Published on: March 31, 2022
Related Concept Videos
Microbial Bioremediation of Pesticides
Production of Biopesticides
Bioreactor Controls-III
Bioremediation
Microbes in the Production of Fermented Foods
iChip