Effects of Biochar Addition and Nitrogen Application Rate on Soil Properties and Agronomic Nitrogen Use Efficiency in...

In modern livestock production, a reliable supply of high-quality forage is essential for sustaining animal productivity and product quality. Although nitrogen (N) fertilization can promote forage growth, excessive N inputs often result in low agronomic nitrogen use efficiency (NAUE) and increased environmental risks. Biochar, owing to its porous structure, high specific surface area, and physicochemical stability, can improve soil physical properties, enhance water and nutrient retention, and regulate soil N availability. However, the mechanisms by which biochar combined with reduced N rate fertilization affects NAUE in artificial grasslands remain insufficiently quantified. A two-year field experiment was conducted at the Grassland Science Experimental Station of Xinjiang Agricultural University on the northern slope of the Tianshan Mountains, Xinjiang, China. Eight treatments were established using a factorial design with two biochar rates (0 and 20 t·ha^-1; B0 and B20) and four N application rates (0, 75, 150, and 225 kg·ha^-1; N0, N75, N150, and N225). Results showed that biochar application significantly decreased soil bulk density and increased soil water content and electrical conductivity. It also elevated soil total carbon, total nitrogen, total phosphorus, NH₄⁺-N, and NO₃^--N concentrations, with B20N150 exhibiting the highest overall nutrient status. Plant community diversity indices did not differ significantly among treatments (p > 0.05), though B20 slightly enhanced Shannon-Wiener and Simpson indices under N0 and N75. Moderate N application significantly increased hay yield, whereas the highest N rate (225 kg·ha^-1) did not further improve yield and reduced NAUE. Biochar combined with N75 or N150 improved NAUE, and B20N150 achieved the best balance of high hay yield and high NAUE. Structural equation modeling revealed that soil water content (path coefficient = 0.45), NH₄⁺-N (0.27), and plant community diversity (0.20) were key positive drivers of NAUE, with biochar exerting indirect effects primarily via improving soil water and available N. Collectively, applying 20 t·ha^-1 biochar with 150 kg·ha^-1 N (B20N150) is recommended as an optimal strategy for N rate reduction and NAUE enhancement in artificial grasslands of arid and semiarid regions.