Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Venous thromboembolism at high altitude: mechanisms, risk stratification, and precision prevention.

Frontiers in physiology·2026
Same author

Self-disgust and its associated factors among patients with malignant lymphoma: a latent profile analysis.

BMC psychology·2026
Same author

Development of Glycosylated and Dimeric FAP-Targeted Radiotracers for Preclinical Evaluation in FAP-Positive Glioblastoma Models.

Journal of medicinal chemistry·2026
Same author

Efficacy and Safety of Thalidomidein Patients with Transfusion-Dependent B-Thalassemia: A Systematic Review, Meta-Analysis and GRADE Evaluation.

Mediterranean journal of hematology and infectious diseases·2026
Same author

Poor platelet engraftment combined with persistent pulmonary infection after haploidentical allogeneic hematopoietic stem cell transplantation: a case report.

Frontiers in medicine·2026
Same author

Pathology-Responsive Nanoprobes for NIR-II Imaging of Acute Kidney Injury.

Analytical chemistry·2026

Related Experiment Video

Updated: Jul 16, 2026

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Triple-Microenvironment Decoding Enables Logic-Unlocked Precision Photoimmunotherapy.

Chuangjun Liu1, Yu Liu2, Simin Liang3

  • 1College of Chemistry and Pharmaceutical Engineering, Huanghuai University, Zhumadian, China.

Angewandte Chemie (International Ed. in English)
|July 15, 2026
PubMed
Summary

This study introduces RPIB-Cys, a novel photosensitizer that precisely targets cancer mitochondria. It activates upon specific stimuli, enabling dual pyroptosis and ferroptosis induction for advanced cancer immunotherapy.

Keywords:
ferroptosismitochondriapyroptosistriple‐locked activationtype‐I photosensitizer

More Related Videos

Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique
05:18

Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique

Published on: March 14, 2016

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
09:32

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

Related Experiment Videos

Last Updated: Jul 16, 2026

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique
05:18

Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique

Published on: March 14, 2016

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
09:32

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Photodynamic Therapy

Background:

  • Current photosensitizers (PSs) for cancer immunotherapy often lack tumor specificity and rely on visible light, limiting their therapeutic precision and effectiveness.
  • Achieving tumor-specific activation of pyroptosis and ferroptosis is crucial for effective cancer immunotherapy, but current methods face limitations in precision and tissue penetration.

Purpose of the Study:

  • To develop a novel near-infrared (NIR) type-I photosensitizer (PS) for precise, mitochondria-targeted activation of pyroptosis and ferroptosis in triple-negative breast cancer (TNBC).
  • To engineer a PS that remains photoinactive until triggered by specific cancer microenvironment conditions, enabling multimodal imaging and dual cell death induction.

Main Methods:

  • Design and synthesis of RPIB-Cys, a self-assembled triple-locked NIR type-I PS.
  • Investigating the cooperative activation mechanism by elevated viscosity, alkaline pH, and high cysteine (Cys) levels in cancer mitochondria.
  • Evaluating the PS's ability to generate reactive oxygen species (ROS), induce ferroptosis (via glutathione depletion and GPX4 inactivation), and pyroptosis (via GSDMD cleavage).
  • Assessing the integration of NIR fluorescence/photoacoustic (PA) imaging with therapeutic effects.

Main Results:

  • RPIB-Cys demonstrated selective photoactivity within the mitochondrial microenvironment of TNBC cells upon stimulation by viscosity, pH, and Cys.
  • The activated PS efficiently generated ROS, leading to concurrent ferroptosis and pyroptosis.
  • This dual immunogenic cell death (ICD) induction was shown to convert 'cold' tumors into 'inflamed' tumors, enhancing the anti-cancer immune response.
  • The PS successfully integrated multimodal imaging capabilities with therapeutic activation.

Conclusions:

  • RPIB-Cys represents a novel, multi-responsive activatable type-I PS for precision cancer immunotherapy.
  • The developed PS uniquely integrates multimodal imaging with the concurrent induction of pyroptosis and ferroptosis, offering a promising strategy for TNBC treatment.
  • This approach facilitates spatially controlled dual ICD, converting non-immunogenic tumors into immunogenic ones for enhanced therapeutic outcomes.