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Updated: Jul 17, 2026

Cost-Efficient Transcriptomic-Based Drug Screening

Cost-Efficient Transcriptomic-Based Drug Screening

Published on: February 23, 2024

Transcriptome Inference and Systematic Approaches to Investigate TCM Compounds With Beneficial Metabolic Effects.

Jing Li^1,2,3, Yang-Chi-Dung Lin^1,2,3, Hsi-Yuan Huang^1,2,3

¹School of Medicine, the Chinese University of Hong Kong, Shenzhen, Guangdong, PR China.

Phytotherapy Research : PTR

|July 15, 2026

Summary

Two traditional Chinese medicine compounds, emodin and triptolide, were identified to combat obesity by enhancing adipose thermogenesis. These compounds boost energy expenditure and reduce fat accumulation, offering potential therapeutic strategies for metabolic disorders.

Keywords:

CMap analysis molecular docking network pharmacology obesity transcriptomic analysis type 2 diabetes mellitus

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Published on: March 3, 2015

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High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

Area of Science:

Metabolic Health
Pharmacology
Traditional Chinese Medicine

Background:

Obesity is a significant risk factor for type 2 diabetes.
Traditional Chinese Medicine (TCM) compounds may improve obesity by increasing energy expenditure.
Identifying novel compounds that promote adipose thermogenesis is crucial for obesity management.

Purpose of the Study:

To identify and validate TCM compounds that promote adipose thermogenesis for obesity alleviation.
To explore the molecular mechanisms underlying the anti-obesity effects of identified TCM compounds.
To utilize a multi-omics approach combining transcriptomics, network pharmacology, and CMap analysis.

Main Methods:

Transcriptomic analysis of 36 TCMs related to adipose thermogenesis.
Network pharmacology and molecular docking for emodin (from Da Huang).
Connectivity Map (CMap) analysis to identify triptolide.
In vitro (3T3-L1 cells) and in vivo (zebrafish) experimental validation.

Main Results:

Emodin and triptolide were identified as potent promoters of adipose thermogenesis.
Both compounds increased the NAD+/NADH ratio, activated AMPK and SIRT1, and suppressed lipid accumulation.
Upregulation of thermogenesis, lipolysis, mitochondrial biogenesis, and beta-oxidation genes observed.
Downregulation of lipogenesis-related genes confirmed anti-obesity effects.

Conclusions:

Emodin and triptolide effectively alleviate obesity by enhancing adipose thermogenesis.
These compounds modulate key metabolic pathways involved in energy expenditure and lipid metabolism.
Combined screening approaches are effective for discovering compounds with beneficial metabolic effects.