Related Experiment Video
Updated: Jul 17, 2026
11:11
Construction and Characterization of a Novel Vocal Fold Bioreactor
Published on: August 1, 2014
Effect of Notch1 on Vocal Fold Re-Epithelization After Acute Injury
Jie Cai1, Susan L Thibeault1, Vlasta Lungova1
1Department of Otolaryngology Head and Neck Surgery, University of Wisconsin Madison, Madison, Wisconsin, USA.
The Laryngoscope
|July 16, 2026
Summary
Notch1 signaling is crucial for vocal fold epithelial repair after injury. Its deletion impairs healing, alters cell proliferation and differentiation, and prolongs inflammation, highlighting its role in coordinating tissue regeneration.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Otolaryngology
Background:
- Vocal fold (VF) epithelial stem cells are vital for tissue repair.
- Notch1 signaling is a key pathway in cellular processes.
- Lrig1 marks a specific population of VF epithelial stem cells.
Purpose of the Study:
- To investigate the function of Notch1 in Lrig1-expressing VF epithelial stem cells.
- To determine Notch1's role in epithelial repair following acute injury.
Main Methods:
- Generated Lrig1CreERT2/+ Notch1F/F mice for conditional Notch1 deletion.
- Induced acute injury using naphthalene (NAPH) in mice.
- Analyzed laryngeal tissues using histology and immunofluorescence at 1, 3, and 7 days post-injury.
Main Results:
- Conditional deletion of Notch1 disrupted VF epithelial repair.
- Observed increased proliferation (Ki-67) and altered stratification (KRT14, KRT13, KRT17).
- Found prolonged inflammation with increased immune cell accumulation (IBA1).
Conclusions:
- Notch1 signaling is essential for regulating epithelial cell fate in VF repair.
- Notch1 coordinates the timing of repair processes in the VF epithelium.
- Disruption of Notch1 impairs healing and prolongs inflammation post-injury.
Related Concept Videos
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
01:28Phases of Wound Repair
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
01:28Tissue Injury: Inflammation and Repair
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...