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Updated: Aug 5, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
Introduction to the Field of Enzyme Immobilization and Stabilization
Michael J Moehlenbrock1, Shelley D Minteer2
1Department of Chemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, MO, 63103, USA.
Abstract:
Enzyme stabilization is important for many biomedical or industrial application of enzymes (i.e., cell-free biotechnology and biosensors). In many applications, the goal is to provide an extended active lifetime at normal environmental conditions with traditional substrates at low concentrations in buffered solutions. However, as enzymes are used for more and more applications, there is a desire to use them in extreme environmental conditions (i.e., high temperatures), in high substrate concentration, and in nontraditional solvent systems. This chapter introduces the topic of enzyme stabilization and the methods used for enzyme stabilization, including enzyme immobilization.
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