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Updated: Aug 6, 2026
High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Published on: August 12, 2019
Synthesis of Large PNA-Encoded Chemical Libraries via Mismatch-Resistant PNA/DNA Hybridization
Jun Hyung Park1, Jungyeon Kim1, Chang Deok Seo1
1Department of Chemistry and Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH), Pohang37673, South Korea.
Abstract:
Peptide nucleic acid (PNA) offers superior chemical stability relative to DNA, enabling encoded library synthesis under conditions incompatible with conventional DNA-based systems. Here, we report a robust PNA encoding strategy for the construction of large single-pharmacophore PNA-encoded libraries through enhanced discrimination between perfectly matched PNA/DNA duplexes and mismatches. This approach integrates a mismatch-destabilizing codon design with optimized hybridization conditions that selectively favor formation of perfectly matched duplexes.
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