Cell-type-specific ATF6α programs regulate epithelial mitochondrial homeostasis and pericyte remodeling during...

Proteostasis declines with lung aging, while the role of the Unfolded Protein Response (UPR) in lung aging and age-associated pulmonary diseases remains understudied. We investigated how deficiency in the UPR sensor ATF6α affects physiological and smoke exposure-accelerated lung aging. ATF6α -deficient mice exhibited accelerated alveolar simplification, a sign of lung parenchymal aging, which was exacerbated by smoking. Nevertheless, small airway vascular fibrotic remodeling, a prominent smoking induced pathology, was not evident in smoke-exposed ATF6α -deficient mice. Mechanistically, these divergent phenotypes arose from cell-type-specific ATF6α programs. In alveolar epithelial type 2 cells (AEC2s), the facultative progenitors of the lung parenchyma, ATF6α maintained mitochondrial bioenergetics and sustained efficient re-differentiation into alveolar epithelial type 1 cells (AEC1s). In lung pericytes, ATF6α promoted extravasation, re-differentiation into myofibroblast-like cells, and production of collagens 1 and 3. These findings identify ATF6α as a cell-type-specific regulator of differentiation programs during lung aging and highlight the need to study ATF6α under defined physiological and pathological contexts before therapeutically targeting this pathway.