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Updated: Aug 6, 2026

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method
Published on: June 13, 2025
[Serial cloners of Yamanashi]
1Biologiste moléculaire, généticien, Président d'Aprogène (association pour la promotion de la génomique), Marseille, France.
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Serial cloning of mice by nuclear transfer has been pursued for 20 years and almost 60 successive generations by a highly dedicated Japanese team. Cloning has been successful up to the 57th generation, although with increasing difficulty from generation 25 onward. Thorough phenotypic and molecular analysis of the successive clones reveals the progressive accumulation of a large number of point mutations and structural variants. This finding has several implications and underlines the necessity of sexual reproduction to ensure continued fitness in successive generations.
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