A novel dual-mode Fluorescence/Colorimetric Aptasensor Based on NH₂-MIL-88B(Fe)/CDs-Apt for Kanamycin Detection

Area of Science:

• Analytical Chemistry
• Materials Science
• Food Science

Background:

• Kanamycin (KANA) is a veterinary antibiotic frequently monitored in food safety testing due to residue concerns.
• Existing detection methods for KANA may lack the sensitivity or speed required for comprehensive food safety analysis.
• Development of rapid, sensitive, and reliable analytical tools is crucial for detecting veterinary drug residues.

Purpose of the Study:

• To introduce a novel metal-organic framework-based aptamer-nanoenzyme (NH2-MIL-88B(Fe)/CDs-Apt) for dual-mode KANA detection.
• To evaluate the sensitivity, accuracy, and applicability of the developed aptasensor in real-world samples.
• To provide an efficient analytical approach for Kanamycin residue monitoring.

Main Methods:

• Fabrication of a composite material using NH2-MIL-88B(Fe) and carbon dots (CDs) functionalized with an aptamer.
• Utilized the catalytic activity of NH2-MIL-88B(Fe) for colorimetric detection via TMB oxidation.
• Employed the fluorescence quenching property of NH2-MIL-88B(Fe) for fluorescent detection of KANA.

Main Results:

• Achieved ultra-low detection limits of 0.716 fM (fluorescent) and 0.709 fM (colorimetric) for KANA.
• Demonstrated successful detection of KANA in complex matrices including eggs, honey, and lake water.
• The aptasensor exhibited high sensitivity, selectivity, and stability in practical applications.

Conclusions:

• The NH2-MIL-88B(Fe)/CDs-Apt aptasensor provides a highly sensitive and accurate platform for dual-mode KANA detection.
• This method holds significant potential for routine food safety testing and environmental monitoring of veterinary drug residues.
• The developed aptasensor offers a promising alternative to conventional analytical techniques for Kanamycin analysis.