Complete genome sequence of Clostridioides difficile NCTC 12727, a PCR ribotype 001 reference strain

Area of Science:

• Microbiology
• Genomics
• Molecular Biology

Background:

• Clostridioides difficile is a significant cause of healthcare-associated infections.
• Understanding the genomic features of specific C. difficile strains is crucial for infection control and treatment strategies.
• PCR ribotyping is a key method for classifying C. difficile strains.

Purpose of the Study:

• To determine the complete genome sequence of the C. difficile PCR ribotype 001 strain NCTC 12727.
• To identify and characterize mobile genetic elements within this specific strain.
• To provide a genomic resource for further research into C. difficile virulence and epidemiology.

Main Methods:

• Hybrid genome assembly was employed for comprehensive sequence data.
• Manual curation was performed to ensure accuracy and completeness of the genome sequence.
• Polymerase Chain Reaction (PCR) validation was utilized to confirm key genomic findings.

Main Results:

• The complete genome sequence of C. difficile NCTC 12727 was successfully obtained.
• The genome is circular and measures 4,371,963 base pairs with a GC content of 28.99%.
• Two multi-copy mobile genetic elements were identified: a prophage and a Tn916-like element.

Conclusions:

• The genome sequence provides a foundational resource for studying C. difficile PCR ribotype 001.
• The identified mobile genetic elements may play a role in the strain's adaptability or virulence.
• This work contributes to the growing understanding of C. difficile genomic diversity.