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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
Protocol for in vivo transfection of mRNA-encapsulated lipid nanoparticles in mouse testis
Liying Wang1, Huafang Wei1, Yiyao Wu1
1Guangzhou Women and Children's Medical Center, Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou 510623, China.
Abstract:
Male infertility affects 10%-20% of the population in most countries, with approximately half of the cases attributed to male factors. Here, we present a protocol for in vivo transfection of mRNA-encapsulated lipid nanoparticles (mRNA-LNPs) into the mouse testis. We describe steps for mRNA-LNP preparation, rete testis delivery, and assessment of transfection and spermatogenic rescue. This protocol establishes a relatively safe and efficient testis transfection system, offering a potential therapeutic strategy for patients with spermatogenic failure.
