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Updated: Aug 6, 2026
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Dissection and Isolation of Mouse Dorsal Root Ganglia
Published on: July 3, 2026
Dissection and Isolation of Mouse Dorsal Root Ganglia
Peixuan Wu1, Wang Ran1, Haifeng Wang2
1Department of Urology, Shanghai East Hospital, School of Medicine, Tongji University.
Journal of Visualized Experiments : Jove
|July 20, 2026
Summary
Researchers developed a standardized protocol for isolating mouse dorsal root ganglia (DRG). This method ensures intact tissue acquisition for studying sensory neurons in pain and neurodegenerative diseases.
Area of Science:
- Neuroscience
- Anatomy
Background:
- Dorsal root ganglia (DRG) are crucial for sensory signal transmission and are vital in pain, nerve injury, and neurodegenerative disease research.
- Acquiring intact DRG tissue is essential for studying sensory neuron properties but presents technical challenges due to their size and location.
Purpose of the Study:
- To develop and present a standardized protocol for the isolation of mouse dorsal root ganglia (DRG).
- To provide detailed anatomical guidance and a reproducible method for obtaining intact DRG tissue.
Main Methods:
- A detailed protocol for mouse DRG isolation, including anatomical localization and vertebral canal exposure.
- The procedure is supported by representative images and video recordings for clarity.
Main Results:
- The developed method enables the acquisition of structurally intact DRG with segmental identification.
- The protocol is suitable for histological analyses like H&E staining and immunofluorescence.
- This method offers standardized recovery, intuitive anatomical guidance, and reduced tissue injury compared to prior techniques.
Conclusions:
- The standardized protocol facilitates the reliable isolation of segmentally identified mouse DRG.
- This method enhances the study of sensory neurons by ensuring high-quality tissue acquisition for various analyses.
