Development of a human melanoma organoid-containing skin-on-a-chip model forin vitrodrug testing and personalized...

Melanoma, an aggressive and lethal form of skin cancer, poses significant challenges in treatment because of its complex biological mechanisms and poor therapeutic response. However, the construction of biomimeticin vitromodels of melanoma with organs-on-a-chip micro-environment have not been reported. This study aimed to design and biofabricate a novel melanoma-containing skin-on-a-chip model for the evaluation of drug responses, and to investigate the effects of ultraviolet (UV) irradiation on melanoma progression. Our results demonstrated pronounced heterogeneity in the tumor immune microenvironment of melanoma patients. The melanoma organoids derived from patients effectively preserve the native immune components, and organoids retained both the histological features and genetic characteristics of the original tumors. Drug response assessments indicated that the combination of dabrafenib and trametinib significantly inhibited melanoma cell proliferation and induced apoptosis; while UV exposure significantly promoted melanoma progression, highlighting the role of environmental factors in tumor development. Our research suggested the biofabrication of a biomimetic melanoma organoid-containing skin-on-a-chip model could elucidate patients' specific melanoma biology, tumor immune microenvironment, and corresponding drug sensitivity.