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Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Rifabutin Cross-resistance and Associated rpoB Mutations in Rifampicin-resistant Mycobacterium tuberculosis Complex
Nalan Sürücüoğlu1, Süheyla Sürücüoğlu2, Nuri Özkütük2
1Clinic of Medical Microbiology, Manisa City Hospital, Manisa, Türkiye.
Thoracic Research and Practice
|July 22, 2026
Summary
Certain rifampicin-resistant Mycobacterium tuberculosis isolates remain susceptible to rifabutin. Identifying specific rpoB mutations can guide treatment selection for drug-resistant tuberculosis.
Area of Science:
- Microbiology
- Genetics
- Pharmacology
Background:
- Rifampicin resistance is a major challenge in treating tuberculosis.
- Rifabutin offers an alternative, but its efficacy in resistant strains requires investigation.
Purpose of the Study:
- To evaluate rifabutin susceptibility in rifampicin-resistant Mycobacterium tuberculosis isolates.
- To identify rpoB gene mutations linked to rifabutin susceptibility.
Main Methods:
- Phenotypic drug susceptibility testing using the MGIT 960 system.
- Sanger sequencing of the rpoB gene's rifampicin resistance-determining region (RRDR).
Main Results:
- 91% of rifampicin-resistant isolates were also rifabutin-resistant.
- Specific rpoB mutations (D516Y, L511P, L533P, M515I+D516Y) were associated with rifabutin susceptibility.
- No mutations were found in rifampicin-susceptible control isolates.
Conclusions:
- Early detection of rpoB mutations can inform rifabutin use in select rifampicin-resistant tuberculosis cases.
- This strategy may improve treatment outcomes and prevent further drug resistance.
- Further in vitro and clinical studies are warranted.
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