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A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

VSIG2 Promotes Osteosarcoma Cisplatin Resistance Via Activating PI3K/AKT/mTOR Pathway

Xueren Zhong1, Jing Xiang2, Xiaoyu Yang1

  • 1Department of Spinal Surgery, Yuebei People's Hospital Affiliated to Shantou University Medical College, Shaoguan, China.

Current Molecular Medicine
|July 23, 2026
PubMed
Abstract

Insights

Vesicle-associated membrane protein A (VAMP) 2 homolog 2 (VSIG2) promotes cisplatin resistance in osteosarcoma (OS). VSIG2 activates the PI3K/AKT/mTOR pathway, offering a potential therapeutic target for overcoming OS chemoresistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Chemotherapy resistance is a major cause of osteosarcoma (OS) relapse.
  • Understanding cisplatin resistance mechanisms is crucial for effective OS therapeutic strategies.

Purpose of the Study:

  • To investigate the role of VSIG2 in mediating chemoresistance in osteosarcoma.
  • To elucidate the molecular pathways involved in VSIG2-driven cisplatin resistance.

Main Methods:

  • In vitro and in vivo experiments including cell viability, colony formation, and xenograft assays.
  • Luciferase reporter assays to identify regulated pathways.
  • Co-immunoprecipitation to determine protein interactions.

Main Results:

  • VSIG2 is upregulated in OS tissues resistant to neoadjuvant chemotherapy.
  • VSIG2 overexpression confers cisplatin resistance in both cellular and animal models.
  • VSIG2 activates the PI3K/AKT/mTOR pathway, leading to increased p70 S6 Kinase phosphorylation.
  • Inhibition of mTOR reverses VSIG2-mediated cisplatin resistance.

Conclusions:

  • VSIG2 plays a significant role in osteosarcoma chemoresistance.
  • VSIG2 confers platinum resistance by activating the PI3K/AKT/mTOR pathway.
  • VSIG2 represents a potential therapeutic target for overcoming chemoresistance in OS.
Keywords:
OsteosarcomaPI3K/AKT/mTOR pathway.VSIG2chemotherapy resistancecisplatin resistance

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