Related Experiment Video
Updated: Aug 10, 2026

07:09
In situ Compressive Loading and Correlative Noninvasive Imaging of the Bone-periodontal Ligament-tooth Fibrous Joint
Published on: March 7, 2014
Ultrasound-Based Estimation of Periodontal Probing Depth Using Category-Specific Supracrestal Tissue Attachment
Mohammadreza Amjadian1, Gulcan Gok1, Nicholas Choi1
1Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, USA.
Dento Maxillo Facial Radiology
|July 24, 2026
Summary
High-frequency ultrasound, calibrated with supracrestal tissue attachment (STA) dimensions, accurately estimates periodontal probing depth (PD) across various teeth. This non-invasive method offers reliable results comparable to traditional probing.
Area of Science:
- Periodontology and Dental Imaging
- Ultrasound technology in dentistry
- Biomaterials and tissue characterization
Background:
- Accurate periodontal probing depth (PD) measurement is crucial for diagnosing and managing periodontal disease.
- Conventional probing can be invasive and subject to inter-examiner variability.
- High-frequency ultrasound offers a non-invasive imaging modality for dental applications.
Purpose of the Study:
- To determine category-specific supracrestal tissue attachment (STA) dimensions using high-frequency ultrasound.
- To calibrate ultrasound measurements to accurately estimate clinical probing depth (PD).
- To assess the reliability of ultrasound landmark annotation in healthy and periodontitis cohorts.
Main Methods:
- Intraoral high-frequency ultrasound (40 MHz) imaging was performed on 34 subjects (20 healthy, 14 periodontitis).
- Four raters annotated 955 teeth for gingival margin (GM), cemento-enamel junction (CEJ), and alveolar bone crest (ABC).
- Ultrasound measurements were calibrated using category-specific STA values to estimate PD, validated against clinical PD using Bland-Altman analysis.
Main Results:
- High inter- and intra-rater reliability (correlation coefficients 0.894–0.998) for ultrasound landmark annotation.
- Calibrated STA values ranged from 1.349 mm to 2.970 mm, aligning with histological data.
- Validation showed 80.8% agreement between ultrasound-derived PD (iPD) and clinical PD within ±1 mm.
Conclusions:
- Category-specific STA-calibrated high-frequency intraoral ultrasound provides clinically acceptable PD estimation.
- This non-invasive approach avoids ionizing radiation and probe-induced discomfort.
- The method demonstrates reliability comparable to conventional periodontal probing, offering a potential adjunct assessment tool.

