Related Experiment Video
Updated: Aug 6, 2026

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Recent advances in the chemoenzymatic synthesis of N- and O-glycans
Shengzhou Ma1, Fangyu Wei2, Liuqing Wen2
1State Key Laboratory of Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Key Laboratory of Marine Drugs of Ministry of Education, Shandong Key Laboratory of Glycoscience and Glycotherapeutics, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Abstract:
Protein N- and O-glycosylation are among the most common and essential post-translational modifications and are implicated in numerous physiological and pathological processes. The efficient assembly of structurally defined N- and O-glycans remains a major challenge due to their structural complexity and diversity. Integrating the flexibility of chemical synthesis and the high regio- and stereospecificity of enzymatic catalysis, chemoenzymatic synthesis represents a powerful strategy for constructing structurally defined N- and O-glycans. This review summarizes recent advances in the chemoenzymatic synthesis of N- and O-glycans, focusing on innovative synthetic strategies and the application of novel enzymes, and offers perspectives for future research directions in this field.
More Related Videos
06:00One-pot Microwave-assisted Conversion of Anomeric Nitrate-esters to Trichloroacetimidates
Published on: January 15, 2018
08:46Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
Published on: July 26, 2018
Related Concept Videos
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Protein Glycosylation
Glycosylation occurs in...
Proteoglycans
Synthesis of α-Substituted Carbonyl Compounds: The Stork Enamine Reaction
Biosynthesis of Polysaccharides
Nitrosation of Enols