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Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
Published on: July 16, 2018
Functional magnetic nanoparticles with delayed chemiluminescence for tyrosinase detection using delay time as a
Chao Hu1, Tianhua Zheng1, Wei Nie1
1State Key Laboratory of Precision and Intelligent Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026, China.
Biosensors & Bioelectronics
|July 24, 2026
Summary
This study introduces a novel chemiluminescence (CL) method using delayed light emission time for tyrosinase (TYR) detection. This approach enhances accuracy and simplifies instrumentation for enzyme activity assays.
Area of Science:
- Analytical Chemistry
- Nanotechnology
- Biochemistry
Background:
- Traditional chemiluminescence (CL) bioassays use flash-type systems with intensity-based quantification, prone to disruptions and requiring sensitive detectors.
- The analytical utility of CL emission time as a quantitative parameter is underexplored, limiting assay development.
- Existing methods face challenges with reproducibility and instrumentation complexity.
Purpose of the Study:
- To develop a novel, time-resolved CL strategy for enzyme activity assays.
- To utilize CL delay time as a quantitative parameter for enhanced detection accuracy and stability.
- To synthesize advanced nanomaterials for improved CL bioassay performance.
Main Methods:
- Synthesis of Mn(II) bifunctional mesoporous silica-coated manganese ferrite core-shell magnetic nanoparticles (MnFe2O4@mSiO2/L012/Mn(Ⅱ) NPs) for delayed CL emission.
- Development of a CL method using CL delay time, coreactants (NaHCO3, H2O2), and a time-regulating reagent (thiourea).
- Application of the method for tyrosinase (TYR) detection in biological samples and inhibitor screening.
Main Results:
- The synthesized NPs exhibited intense, delayed CL emission with a narrow full width at half maximum (0.61 s), attributed to high L012 and Mn(II) loading.
- The CL delay time was tunable, enabling precise, time-resolved measurements with robust magnetic separability and long-term stability.
- A detection limit of 26 U/L for TYR was achieved, demonstrating improved accuracy and simplified instrumentation compared to intensity-based methods.
Conclusions:
- A novel time-resolved CL strategy using CL delay time as a quantitative parameter was successfully developed.
- The developed method offers improved accuracy, stability, and simplified instrumentation for enzyme activity assays.
- The strategy shows significant potential for tyrosinase detection in human serum and tyrosinase inhibitor screening, applicable to broader biomedical applications.
