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Working with Auditory HEI-OC1 Cells

Working with Auditory HEI-OC1 Cells

Published on: September 3, 2016

Epac1 Alleviates Senescence in Auditory Hair Cells via the Ferroptosis

Wen-Jun An^1,2, Chen-Yu Xu¹, Meng-Ya Shen¹

¹Departments of Otolaryngology - Head and Neck Surgery, The First Affiliated Hospital of USTC Division of Life Science and Medicine, University of Science and Technology of China Hefei China.

World Journal of Otorhinolaryngology - Head and Neck Surgery

|July 25, 2026

Abstract

Objectives:

The aim of this study was to investigate the changes in Epac1 and ferroptosis-related proteins in aged mice and the House Ear Institute-Organ of Corti 1 (HEI-OC1) cells, with the goal of elucidating their potential role in age-related hearing loss.

Methods:

The expression of Epac1 in the cochlea of C57BL/6J mice was examined by using immunofluorescence staining and Western blot analysis. To further verify the role of Epac1 in age-related hearing loss, small interfering RNA (siRNA) was transfected into HEI-OC1 cells, resulting in the downregulation of Epac1.

Results:

The results revealed a decrease in Epac1 expression in the cochlea of aged mice. After downregulating the level of Epac1 in vitro, a greater level of ferroptosis was observed, and the aging process was accelerated.

Conclusions:

Epac1 plays a crucial role in the aging process of auditory hair cells via ferroptosis.

Keywords:

age‐related hearing loss auditory hair cell ferroptosis

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