Draft genome sequence of Pseudonocardia alni M29 isolated from healthy maize plant

Area of Science:

• Microbiology
• Genomics
• Plant Science

Background:

• The genus Pseudonocardia produces siderophores crucial for plant iron uptake.
• Endophytic microorganisms reside within plant tissues, influencing plant health and nutrient acquisition.

Purpose of the Study:

• To report the draft genome sequence of Pseudonocardia alni M29, an endophyte isolated from maize.
• To provide genomic insights into Pseudonocardia alni's potential for siderophore production and plant-microbe interactions.

Main Methods:

• Isolation of Pseudonocardia alni M29 from healthy maize plants.
• De novo genome assembly using shotgun sequencing.
• Gene prediction and annotation using KEGG and CAZy databases.
• Genome completeness assessment using CheckM and BUSCO.

Main Results:

• The draft genome of Pseudonocardia alni M29 has a size of 5.96 Mb, assembled into 53 contigs.
• 5525 protein-coding genes were predicted, with significant annotation in KEGG and CAZy databases.
• Genome analysis indicated high completeness (98.87%) with minimal gene fragmentation or loss.

Conclusions:

• The availability of the Pseudonocardia alni M29 genome provides a valuable resource for studying plant-associated bacteria.
• Genomic data can elucidate the mechanisms of siderophore-mediated iron uptake in maize-endophyte symbioses.