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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Subgingival Microbial Profiles at Peri-Implant and Adjacent Tooth Sites: A Cross-Sectional Quantitative PCR Study
Ioana Suciu1, Simona Ruță1,2,3, George Suciu4
1Romanian Academy, School of Advanced Studies of the Romanian Academy (SCOSAAR), Calea 13 Septembrie 13, 050711 Bucharest, Romania.
Dentistry Journal
|July 27, 2026
Summary
Peri-implantitis sites show higher bacterial loads for specific bacteria compared to adjacent teeth, indicating distinct microbial profiles. Healthy implants and teeth had similar microbial communities, suggesting localized inflammation drives peri-implantitis.
Area of Science:
- Microbiology
- Periodontology
- Dental Implantology
Background:
- Peri-implant diseases stem from microbial biofilm on implant surfaces.
- This can lead to inflammation, bone loss, and potential implant failure.
Purpose of the Study:
- Analyze subgingival microbial profiles in peri-implantitis (PI) and adjacent teeth (TT).
- Assess the influence of smoking and periodontal status on these microbial profiles.
Main Methods:
- Cross-sectional study of 43 adults with 100 implants and 50 teeth.
- Subgingival biofilm analyzed via quantitative PCR (Paro x panel) for 22 bacterial taxa.
- Sites classified as healthy implants (HI), peri-implantitis (PI), periodontally diseased (TT), or healthy (TT).
Main Results:
- PI sites had significantly higher bacterial loads for 10 taxa, including *Campylobacter rectus* and *Porphyromonas gingivalis*, compared to TT.
- No significant differences in bacterial load between HI and TT sites.
- Higher prevalence of bleeding on probing and suppuration observed in PI sites versus HI sites.
Conclusions:
- Peri-implant and adjacent tooth sites share microbial overlap.
- Peri-implantitis is associated with increased detection and bacterial loads of specific taxa.
- Microbial profiles of healthy implants and teeth are less distinct.
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