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AI-Assisted Forensic Analysis of Hanging-Related Ligature Marks: A Pilot Study Using Convolutional Neural Networks
Giorgia Rigano1, Fabrizio De Vita2, Lucia Candela1
1Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Via Consolare Valeria, 1, 98125 Messina, Italy.
Diagnostics (Basel, Switzerland)
|July 28, 2026
Summary
Artificial intelligence (AI) shows promise in analyzing forensic images. A deep learning model accurately classified hanging-related ligature marks, aiding forensic pathology assessments.
Area of Science:
- Forensic pathology
- Medical image analysis
- Artificial intelligence
Background:
- AI application in forensic pathology is limited.
- Assessing ligature marks in hanging deaths is challenging for forensic experts.
- This study pilots a deep learning approach for classifying these marks.
Purpose of the Study:
- To evaluate a deep learning approach for morphological classification of hanging-related ligature marks.
- To establish a framework for AI-assisted forensic image analysis.
Main Methods:
- A Convolutional Neural Network (CNN) was trained on 404 standardized JPEG images.
- The dataset included hanging-related ligature marks, strangulation, and post-mortem artefacts.
- The model was validated internally and tested on independent case images from Italy and Lithuania.
Main Results:
- The CNN achieved an F1-score of 0.81 ± 0.04 in distinguishing hanging-related ligature marks.
- The study established criteria for image standardization in AI-assisted forensic analysis.
- The model showed encouraging performance in classifying lesions.
Conclusions:
- AI-based image analysis can potentially assist in evaluating ligature marks during external examinations.
- Forensic diagnosis necessitates integrating AI findings with autopsy, physical examination, and circumstantial evidence.
- Further research with larger datasets and multicenter protocols is required to confirm reliability and applicability.