Ce3Light: Design, Construction, and Testing of a Light-Irradiation System for In Vitro Cell Studies

Photobiomodulation (PBM) has gained increasing attention in tissue engineering and cell biology, yet reproducible in vitro studies remain limited by the availability of standardized irradiation platforms. This study presents the design, construction, and technical validation of Ce3Light, a modular LED-based irradiation system developed for controlled photobiomodulation experiments under standard cell culture conditions. The platform was fabricated using additive manufacturing (PA12) and incorporates interchangeable LED modules, integrated monitoring of illuminance and temperature, and compatibility with conventional CO₂ incubators. To demonstrate its applicability for in vitro research, the system was evaluated using MRC-5 fibroblasts exposed to four wavelengths (460, 530, 660, and 800 nm). Cellular responses were assessed by measuring intracellular glutathione levels and dehydrogenase activity. The experiments confirmed that the platform enabled stable and reproducible irradiation while detecting wavelength-dependent cellular responses consistent with previous reports in the literature. These findings primarily validate the functionality and suitability of Ce3Light as an experimental platform rather than establish new biological mechanisms. The developed system provides a versatile, reproducible, and adaptable tool for future photobiomodulation studies requiring precise control of irradiation conditions.