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Updated: Aug 5, 2026

Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling
Published on: December 10, 2019
Comparative Analysis of Volatile Profiles from Rosa gallica L. Flowers Using HS and SPME Extraction Coupled with
1College of Tea and Food Science, Xinyang Normal University, Xinyang 464000, China.
Abstract:
Volatile composition is a core trait that determines the ornamental and application value of Rosa flowers. In the current study, the volatile profiles of freeze-dried Rosa gallica flowers were comprehensively characterized via gas chromatography-mass spectrometry (GC-MS), coupled with heatmap clustering, principal component analysis (PCA), and relative odor activity value (rOAV) determination. A total of 181 volatile compounds were identified, and distinct volatile profiles were observed between flower samples treated with headspace extraction (RG-HS) and solid-phase microextraction (RG-SPME). Alcohols were the dominant volatile class in RG-SPME, whereas aldehydes were abundant in the RG-HS group. In terms of aroma characteristics, RG-SPME presented stronger green, floral, citrus, and fatty odors, while RG-HS exhibited a more pronounced fruity note. Moreover, sulfurous and dairy notes were unique to RG-SPME and RG-HS, respectively. Additionally, 27 volatiles showed rOAV above one, with 2,3-butanedione and trans-citral having the greatest rOAV value of 100, were identified as the potential aroma-active contributors to butter-like and citrus odors in RG-HS and RG-SPME, respectively. These results provide a methodological reference for volatile profiling of R. gallica flowers, and support the selection of appropriate extraction methods for future research and resource utilization.
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