Systemic Molecular Network Alterations Associated with FoxA2 in Gastric Cancer

Area of Science:

• Oncology
• Molecular Biology
• Biochemistry

Background:

• Gastric cancer involves complex interactions in transcriptional regulation, inflammation, metabolism, and angiogenesis.
• Systemic molecular links between these processes in gastric cancer are not fully understood.

Purpose of the Study:

• To evaluate circulating levels of FOXA2, Ang-1, ApoE4, PEN-2, and NF-κB in gastric cancer patients.
• To explore the integrated role of these molecules in gastric cancer biology and their potential as biomarkers.

Main Methods:

• Serum samples were collected from 40 gastric cancer patients (20 preoperative, 20 postoperative) and 20 healthy controls.
• Protein levels of FOXA2, Ang-1, ApoE4, PEN-2, and NF-κB were quantified using enzyme-linked immunosorbent assay (ELISA).
• Statistical analyses were performed to compare protein levels between groups.

Main Results:

• Serum levels of FOXA2, ApoE4, PEN-2, and NF-κB were significantly decreased in gastric cancer patients versus controls.
• Serum levels of Ang-1 were significantly increased in gastric cancer patients compared to controls.
• No significant differences were found between preoperative and postoperative gastric cancer groups.

Conclusions:

• Gastric cancer exhibits coordinated dysregulation of transcriptional, inflammatory, metabolic, and angiogenic processes.
• The identified FOXA2-NF-κB-PEN-2-ApoE4-Ang-1 axis represents an integrated circulating molecular profile.
• This profile may serve as a basis for developing non-invasive biomarkers and advancing translational research in gastric cancer.