Association Between Single-Nucleotide Polymorphisms and DRAM1 Gene Expression in Periodontal Ligament Fibroblasts...

Area of Science:

• Genetics
• Cell Biology
• Orthodontics

Background:

• Autophagy is crucial for orthodontic tooth movement.
• DNA damage-regulated autophagy modulator 1 (DRAM1) regulates autophagic cargo degradation.
• DRAM1 expression is differentially regulated in human periodontal ligament (hPDL) fibroblasts under compressive force.

Purpose of the Study:

• To investigate the impact of DRAM1 single-nucleotide polymorphisms (SNPs) on DRAM1 gene expression in hPDL fibroblasts under compressive force.
• To determine if specific DRAM1 SNPs influence gene expression during simulated orthodontic tooth movement.

Main Methods:

• hPDL fibroblasts from 59 patients were subjected to a compressive strain of 2 g/cm³.
• Relative DRAM1 gene expression was measured under loaded and control conditions.
• Genotyping analysis of six DRAM1 SNPs (rs756534, rs2138257, rs2176092, rs4622329, rs10860812, rs4764657) was performed using real-time PCR.
• Linear regression was used to evaluate SNP-SNP interactions.

Main Results:

• No statistically significant differences in relative DRAM1 gene expression were observed among the genotypes of the studied SNPs (p > 0.05).
• SNP-SNP interaction analysis did not reveal any statistically significant associations.
• The tested intronic SNPs within the DRAM1 gene did not show a regulatory role in gene expression under orthodontic compression.

Conclusions:

• The expression of DRAM1 in hPDL fibroblasts under orthodontic compression is not significantly regulated by the investigated intronic SNPs.
• These findings suggest that other regulatory mechanisms may be involved in DRAM1 modulation during orthodontic tooth movement.
• Further research is needed to explore other genetic variants or regulatory factors influencing DRAM1 in this context.