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Development and Optimization of Antioxidant Poly Herbal Peel-off Mask using Doe Based Approach
Devika Ramakrishnan1, Jithesh Mr1, Jisna Joy1
1Department of Pharmaceutics, Nirmala College of Pharmacy, Muvattupuzha, Kerala, India.
Background:
Antioxidants protect the skin from free radicals, which are associated with signs of aging, pigmentation, and loss of elasticity. Combining antioxidants in cosmetic formulations may provide broader antioxidant potential. This study focuses on the formulation and optimization of a polyherbal antioxidant peel-off mask using extracts of Ocimum sanctum (Tulsi), Moringa oleifera (Moringa), and Trigonella foenum-graecum (Fenugreek), which are reported to possess antioxidant and skin-beneficial properties.
Methodology:
The formulation and optimization of the polyherbal peel-off mask were carried out using Central Composite Design (CCD). Polyvinyl alcohol (PVA) and carbomer 940 served as film-forming agents, while triethanolamine was used to adjust viscosity. The optimized formulation was evaluated for physicochemical characteristics, in vitro antioxidant activity, and irritation potential using the HET-CAM assay.
Results:
The optimized formulation exhibited a drying time of 25.3 minutes and an applicability score of 18.82. Physicochemical evaluation showed acceptable homogeneity, smooth texture, and pH suitable for topical application. In vitro antioxidant assays demonstrated a lower IC50 value for the polyherbal mask compared to the standard vitamin C formulation, indicating notable antioxidant activity. Furthermore, the CAM (chorio-allantoic membrane) irritation test showed no observable signs of irritation such as hemorrhage, lysis, or coagulation under the study conditions.
Conclusion:
This study successfully formulated and optimized a polyherbal peel-off mask with promising in vitro antioxidant activity and acceptable physicochemical characteristics. The use of Central Composite Design (CCD) enabled systematic optimization of the formulation variables. The findings suggest the potential applicability of the developed herbal formulation for cosmetic skincare applications; however, further stability studies and in vivo safety and efficacy evaluations are required.
