Rutin attenuates acrylamide-induced oxidative liver injury

Area of Science:

• Toxicology
• Hepatology
• Pharmacology

Background:

• Acrylamide (ACR) is a toxic industrial chemical with confirmed in vitro and in vivo effects.
• Limited research exists on ACR-induced liver injury, necessitating further investigation.
• Rutin (Rut), a plant-derived flavonoid, possesses known pharmacological properties.

Purpose of the Study:

• To investigate the therapeutic potential of Rut in mitigating ACR-induced hepatic injury in rats.
• To explore the mechanistic basis of Rut's protective effects against ACR-induced liver damage.

Main Methods:

• Fifty male Sprague-Dawley rats were divided into five groups: Control, ACR, and three Rut + ACR groups (100, 200, 400 mg/kg).
• Intervention lasted 21 days, with daily monitoring of body weight and liver coefficient.
• Biochemical assays (ALT, AST, SOD, GSH, MDA) and histopathological analyses (H&E, Masson staining, IHC for TNF-α and cleaved caspase-3) were performed.

Main Results:

• ACR exposure significantly increased liver injury markers (ALT, AST, MDA) and decreased protective factors (SOD, GSH), while Rut administration dose-dependently reversed these changes.
• Histopathology revealed reduced necrosis and collagen deposition in Rut-treated rats.
• Rut suppressed ACR-induced increases in TNF-α and cleaved caspase-3 expression.

Conclusions:

• Rutin demonstrates significant protective effects against acrylamide-induced liver injury in rats.
• Rut ameliorates ACR-induced hepatic damage by reducing oxidative stress and inflammation.
• Rutin may serve as a potential preventative agent for acrylamide-induced liver injury.