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Updated: Aug 5, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Lactococcus cremoris subsp. sagaensis subsp. nov., Isolated from Naturally Fermented Yogurt
Lin Cheng1, Tian-Xue Wang1, Chun Yan Li2
1College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
Abstract:
Four lactic acid bacteria, designated 1030T, 1245, X2047 and 5 - 2, from naturally fermented yogurt in Xizang Autonomous Region, PR China, were characterized using a polyphasic approach. The results of 16 S rRNA gene, pheS, recA and rpoB sequence analyses and phylogenomic tree indicated that strains 1030T, 1245, X2047 and 5 - 2 were most closely related to the type strains of Lactococcus cremoris subsp. cremoris and L. cremoris subsp. tructae. Strains 1030T, 1245, X2047 and 5 - 2 shared 97.8-98.1% ANI and 81.0-83.1% dDDH values with L. cremoris subsp. cremoris ATCC 19257T and L. cremoris subsp. tructae DSM 21502T, indicating that they belonged to L. cremoris. However, phenotypic difference between strains 1030T, 1245, X2047, 5 - 2 and L. cremoris subsp. cremoris and L. cremoris subsp. tructae was large. Based upon the data obtained in the present study, a novel subspecies, L. cremoris subsp. sagaensis subsp. nov., is proposed and the type strain is 1030T (= JCM 38217T=GDMCC 1.6009T).
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