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Published on: November 29, 2024
Stable Isotope-Resolved Metabolomics in Elucidating Mitochondrial Metabolic Reprogramming and Therapeutic Targets
Dan Yang1, Xinyan Wu2, Xiufang Luo3
1Engineering Center of Agricultural Biosafety Assessment and Biotechnology, School of Advanced Agricultural Sciences, Yibin Vocational and Technical College, Yibin, China.
Biomedical Chromatography : BMC
|July 31, 2026
Summary
Stable isotope-resolved metabolomics (SIRM) reveals how mitochondrial metabolism changes in diseases like cancer and Alzheimer's. This technique tracks metabolic pathways to identify new therapeutic targets and monitor treatment effectiveness.
Area of Science:
- Biochemistry
- Systems Biology
- Metabolomics
Background:
- Mitochondrial metabolic reprogramming is crucial in diseases.
- Stable isotope-resolved metabolomics (SIRM) offers quantitative insights into metabolic flux.
- Understanding these pathways is key for developing targeted therapies.
Purpose of the Study:
- To review the application of SIRM in understanding disease-associated metabolic reprogramming.
- To highlight SIRM's role in identifying therapeutic targets and monitoring treatments.
- To discuss advancements, challenges, and future directions in SIRM for metabolic therapies.
Main Methods:
- Utilizes isotopically labeled substrates to trace metabolic pathways.
- Combines stable isotope tracing with metabolic flux analysis (MFA).
- Applies SIRM to analyze mitochondrial carbon flux in various disease models.
Main Results:
- SIRM has elucidated metabolic reprogramming signatures in cancer, neurodegenerative disorders, and metabolic syndrome.
- Identified key metabolic dependencies and elucidated drug mechanisms.
- Demonstrated SIRM's utility in therapeutic monitoring and resistance assessment.
Conclusions:
- SIRM is a powerful tool for dissecting mitochondrial metabolism in disease.
- It provides quantitative biomarkers for patient stratification and treatment optimization.
- Future innovations, including AI, will further enhance SIRM's impact on metabolism-targeted therapies.
