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Updated: Aug 5, 2026
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
Published on: March 8, 2019
Protocol for the enrichment of endosteal and periosteal mesenchymal cells from murine bone for single-cell
Alongkorn Kurilung1, Visanu Wanchai1, Jinhu Xiong2
1Center for Musculoskeletal Disease Research, University of Arkansas for Medical Sciences, Little Rock, AR, USA; Department of Biomedical Informatics, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Abstract:
Bone comprises diverse cell populations essential for skeletal development, remodeling, and homeostasis. Current single-cell isolation methods often use bulk digestion that can obscure distinctions between periosteal and endosteal compartments. Here, we present an optimized murine bone single-cell RNA sequencing (scRNA-seq) workflow using Liberase™ digestion, EDTA decalcification, and sequential depletion of hematopoietic and endothelial cells to improve yield and viability. We also provide a basic Seurat (R) pipeline for data processing and visualization. For complete details on the use and execution of this protocol, please refer to Nookaew et al.1.
