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Tannic acid-crosslinked zein-lipase complex stabilized multichamber Pickering emulsion for efficient diacylglycerol
Zhouliang Sun1, Yufei Zhang2, Ji Zhang1
1Hubei Hongshan Laboratory, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Wuhan 430062, China; College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Abstract:
This study presents a food-grade, multi-chamber W/O/W high internal phase Pickering emulsion (HIPPE) system for interfacial catalysis, which stabilized by a zein nanoparticle (ZNP)-lipase complex crosslinked with tannic acid (TA). Experiments and molecular dynamics simulations confirmed that the cross-linking between TA, ZNPs, and lipase was predominantly mediated by hydrogen bonding, hydrophobic interactions, and π-π stacking. TA effectively modulated the zein-lipase interaction to form a stable, dispersed complex and substantially enhanced the thermal stability of the lipase. The multi-chambered W/O/W HIPPE was successfully applied to hydrolyze natural vegetable oils for producing DAG-enriched edible oils without external stirring during the reaction. Using only 0.3 wt% lipase with soybean oil at 40 °C, we produced a functional oil containing 43.3% DAG within 60 min. The system also demonstrated broad applicability to various oils, such as rapeseed oil and flaxseed oil. This system establishes a robust, sustainable platform for efficient green synthesis.
