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Protein Modification via Bioorthogonal HS-BCN Ligation

Ming Fang1, Qing Lin2

  • 1Department of Chemistry, State University of New York at Buffalo, Buffalo, NY, 14260-3000, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 1, 2026
PubMed
Summary

The hydrazonyl sultone (HS)-bicyclo[6.1.0]non-4-yne (BCN) ligation enables precise protein modification. This bioorthogonal reaction is effective for labeling nanobodies and G protein-coupled receptors (GPCRs) in vitro and in live cells.

Keywords:
BioconjugationBioorthogonal chemistryClick chemistryG protein-coupled receptorHydrazonyl sultoneNanobody

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