Host-Specific Interactions Between Staphylococcus aureus and Pseudomonas aeruginosa Impair Epithelial Repair in
Xiaohan Sun1,2, Mahnaz Ramezanpour1,2, George Bouras1,2
1Department of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network, Woodville, South Australia, Australia.
Background:
Chronic rhinosinusitis (CRS) is frequently associated with polymicrobial biofilms involving Staphylococcus aureus and Pseudomonas aeruginosa. Interactions between these organisms are thought to influence disease severity, but the epithelial effects of exoproteins derived from patient-matched cocultures remain poorly defined.
Methods:
Clinical isolates of S. aureus and P. aeruginosa (n = 3 each) co-isolated from three CRS patients were cultured in a Transwell system as same-patient or cross-patient pairs. Cell-free exoproteins were applied to primary human nasal epithelial cells. Epithelial repair was assessed using a scratch assay, while cytotoxicity, oxidative stress, and inflammatory responses were evaluated by lactate dehydrogenase release, intracellular reactive oxygen species measurement, and interleukin-6 secretion. Exoprotein profiles were further characterized using data-independent acquisition proteomics.
Results:
Exoproteins derived from same-patient cocultures consistently impaired epithelial wound closure compared with P. aeruginosa monocultures, although the timing of inhibition varied among patients. These exoproteins also induced greater epithelial cytotoxicity, elevated intracellular reactive oxygen species levels, and increased interleukin-6 secretion compared with monocultures or cross-patient cocultures. In contrast, cross-patient pairings produced epithelial responses similar to monoculture conditions. Proteomic analysis indicated that patient origin was a major determinant of exoproteome organization, with same-patient cocultures showing increased abundance of proteins associated with redox balance and metabolic regulation.
Conclusions:
Patient-matched S. aureus-P. aeruginosa interactions were associated with increased epithelial stress and inflammatory responses, together with differences in the exoproteomic profile. These findings suggest that host-specific bacterial interactions may contribute to virulence and possibly recalcitrance in CRS.
Insights
Interactions between Staphylococcus aureus and Pseudomonas aeruginosa from the same chronic rhinosinusitis patient worsen epithelial healing and increase inflammation. These patient-specific bacterial interactions may drive disease severity.
Area of Science:
- Microbiology
- Immunology
- Cell Biology
Background:
- Chronic rhinosinusitis (CRS) often involves polymicrobial biofilms, including Staphylococcus aureus and Pseudomonas aeruginosa.
- The impact of exoproteins from patient-matched bacterial cocultures on nasal epithelium is not well understood.
Purpose of the Study:
- To investigate the effects of exoproteins from same-patient and cross-patient cocultures of S. aureus and P. aeruginosa on human nasal epithelial cells.
- To characterize the exoproteomic differences associated with these interactions.
Main Methods:
- Co-culturing clinical isolates of S. aureus and P. aeruginosa from CRS patients.
- Applying cell-free exoproteins to primary human nasal epithelial cells.
- Assessing epithelial repair, cytotoxicity, oxidative stress, and inflammation (IL-6).
- Proteomic analysis of exoprotein profiles.
Main Results:
- Exoproteins from same-patient cocultures significantly impaired epithelial wound closure, increased cytotoxicity, and elevated oxidative stress and IL-6 secretion compared to monocultures or cross-patient cocultures.
- Cross-patient coculture exoproteins yielded responses similar to monocultures.
- Proteomics revealed distinct exoproteome organization in same-patient cocultures, with increased proteins related to redox balance and metabolism.
Conclusions:
- Patient-specific interactions between S. aureus and P. aeruginosa exoproteins heighten epithelial stress and inflammation.
- These host-specific bacterial interactions may contribute to the virulence and persistence of chronic rhinosinusitis.
More Related Videos
07:42Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition
Published on: September 3, 2016
09:02Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
Published on: September 22, 2023
Related Concept Videos
01:29Microbiota of the Respiratory Tract
01:29Staphylococcal Skin Infections
01:28Gene Regulation in Microbial Communities: Quorum Sensing
01:25Clinical Significance of Antibiotic Resistance