Related Experiment Video
Updated: Aug 5, 2026
11:13
Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Human TBC1 domain-containing kinase is a class I multidomain pseudokinase
Shiwangi Maurya1, Lilly E Cheek1, Anthony T Iavarone2
1Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, 32306, USA.
Protein Expression and Purification
|August 3, 2026
Summary
TBCK-related encephalopathy (TBCKE) is a neurodevelopmental disorder. Researchers purified human TBCK protein, finding its pseudokinase domain lacks nucleotide binding, suggesting it
Area of Science:
- Biochemistry
- Molecular Biology
- Neuroscience
Background:
- TBCK-related encephalopathy (TBCKE) is a neurodevelopmental disorder caused by mutations in the TBCK gene.
- The biochemical and biophysical properties of TBCK protein are not well understood, limiting insight into TBCKE.
Purpose of the Study:
- To express and purify full-length human TBCK protein.
- To characterize the biochemical and biophysical properties of TBCK.
- To understand the molecular basis of TBCK's role in TBCKE.
Main Methods:
- Expression of full-length human TBCK in Spodoptera frugiperda cells.
- Purification of recombinant TBCK protein.
- Biochemical and biophysical analyses, including nucleotide binding assays.
Main Results:
- Successfully expressed and purified full-length human TBCK.
- The pseudokinase domain of TBCK is catalytically inactive.
- TBCK lacks nucleotide binding due to the absence of key catalytic motifs (VAIK, HRD, DFG).
Conclusions:
- TBCK functions as a class I pseudokinase.
- These findings provide a foundation for future studies on TBCK's structure and function in TBCKE.
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