Related Experiment Video
Updated: Aug 5, 2026
09:56
A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy
Published on: February 21, 2025
Bispecific ANXA2/CD147 CAR-T cell therapy for osteosarcoma
Hai-Jun Tang1, Wei Dai1, Dan-Ting Xiao1
1Department of Spine and Osteopathic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Oncogene
|August 3, 2026
Summary
A new bispecific CAR-T cell therapy targeting ANXA2 and CD147 shows enhanced efficacy against osteosarcoma. This novel approach offers improved antitumor activity and a promising strategy for osteosarcoma immunotherapy.
Area of Science:
- Oncology
- Immunotherapy
- Cellular Therapy
Background:
- Osteosarcoma is a highly malignant bone cancer with a poor prognosis.
- Current single-target CAR-T cell therapies for osteosarcoma have limited efficacy.
Purpose of the Study:
- To develop and evaluate a novel bispecific CAR-T cell therapy targeting ANXA2 and CD147 for osteosarcoma.
- To provide an experimental basis for improving osteosarcoma treatment outcomes.
Main Methods:
- Single-cell RNA sequencing (scRNA-seq) identified ANXA2 and CD147 as osteosarcoma antigens.
- A bispecific ANXA2/CD147 CAR-T construct was generated and validated.
- In vitro cytotoxicity assays and in vivo xenograft models (CDX, PDX) were used to assess efficacy.
Main Results:
- Bispecific CAR-T cells demonstrated significantly greater cytotoxicity and released higher levels of effector molecules (GZMB, TNFα) compared to single-target controls.
- In vivo studies showed superior antitumor activity, reduced tumor growth, and decreased metastasis.
- Increased infiltration of M1 macrophages was observed in tumors treated with bispecific CAR-T cells.
Conclusions:
- A bispecific ANXA2/CD147 CAR-T cell was successfully generated with potent antitumor activity.
- This novel CAR-T cell therapy presents a promising new strategy for osteosarcoma immunotherapy.
Related Concept Videos
01:27Tumor Immunotherapy
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
02:57Targeted Cancer Therapies
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
There are several types of targeted therapies against specific...
02:49Cancer Therapies
Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...