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Updated: Aug 5, 2026
A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
Metal-enhanced fluorescent sensor system for bisphenol a determination using AgNP-modified molecularly imprinted
Daria Yarynka1, Roman Nikolaiev1, Oleksandr Brovko2
1Institute of Molecular Biology and Genetics of the NAS of Ukraine Zabolotnogo Str. 150 Kyiv 03143 Ukraine d.v.yarynka@nas.gov.ua.
Abstract:
A metal-enhanced fluorescence sensor system was developed for the detection of bisphenol A (BPA) using molecularly imprinted polymer (MIP) films with in situ synthesized silver nanoparticles (AgNPs). The sensor design combines the high selectivity of ethylene glycol methacrylate phosphate (EGMP)-containing MIP films and the fluorescence amplification capabilities of AgNPs via metal-enhanced fluorescence (MEF) phenomenon. Analytical characteristics were evaluated, including detection limit, linear dynamic range and selectivity. The proposed MEF sensor demonstrated a detection limit of 0.1 nM for BPA and a linear dynamic range of 0.1 nM-25 µM. The thin AgNP-MIP films immobilized on glass slides exhibited significantly higher selectivity toward BPA compared to its structurally related analogues (2-, 3-, 4-nitrophenols, phenol, o- and p-cresols, resorcinol, catechol). The MEF sensor system was successfully applied to detect BPA in spiked wastewater samples, demonstrating potential for robust and sensitive environmental monitoring.
