Platelet flow cytometry: An evolving platform for clinical hematology

Area of Science:

• Hematology
• Immunology
• Cellular Biology

Background:

• Flow cytometry offers high-resolution, single-cell analysis of platelets.
• It has evolved from basic research to clinical diagnostics.

Purpose of the Study:

• To review the evolution and expanding applications of flow cytometry in platelet biology and diagnostics.
• To highlight its role in various immune and thrombotic disorders.

Main Methods:

• Review of literature tracing the historical development of flow cytometry in platelet studies.
• Discussion of advanced techniques like spectral cytometry, phosphoflow, and fluorescent barcoding.
• Examination of clinical applications and emerging capabilities.

Main Results:

• Flow cytometry is crucial for analyzing platelet surface markers, activation, and signaling.
• It plays key roles in diagnosing conditions like immune thrombocytopenia, HIT, VITT, and FNAIT.
• Advanced methods enable detailed characterization of platelet subpopulations and function.

Conclusions:

• Flow cytometry is integral to modern platelet research and diagnostics.
• Its capabilities support risk assessment, quality control, and personalized hematology.
• The technology is expanding into clinical workflows for more informed patient care.