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Updated: Aug 7, 2026
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Inducing Apical Periodontitis in Mice
Published on: August 6, 2019
CD68 and F4/80 Markers Demonstrate Inflammatory Synergism Between Apical Periodontitis and Liver Fibrosis in Wistar
L V Barroti1, C Cantiga-Silva1, M P Justo1
1Endodontic Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, SP, Brazil.
International Endodontic Journal
|August 6, 2026
Summary
Apical periodontitis and liver fibrosis worsen together, increasing macrophage markers CD68 and F4/80. This study in Wistar rats shows inflammatory synergy between these conditions.
Area of Science:
- Immunology and Pathology
- Oral Biology
- Hepatology
Background:
- Apical periodontitis (AP) and liver fibrosis (LF) are distinct inflammatory conditions.
- Macrophage markers CD68 and F4/80 are implicated in inflammatory processes.
Purpose of the Study:
- To investigate the combined effect of AP and LF on Wistar rats.
- To evaluate the expression of CD68 and F4/80 glycoproteins in co-existing AP and LF.
Main Methods:
- Wistar rats were divided into control, AP, LF, and AP+LF groups.
- LF was induced using carbon tetrachloride and bile duct ligation.
- AP was induced by pulp exposure; histological and immunohistochemical analyses were performed.
Main Results:
- Co-infection with AP and LF showed increased portal inflammation and collagen deposition in the liver.
- Both liver and periapical tissues exhibited higher CD68 and F4/80 expression in the AP+LF group compared to single conditions.
- Necrosis and inflammatory infiltrate were observed in the dental pulp and periapical tissues of AP and AP+LF groups.
Conclusions:
- CD68 and F4/80 glycoproteins demonstrate inflammatory synergy between AP and LF.
- The co-occurrence of AP and LF significantly increases macrophage infiltration in both affected tissues.
